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PET28 Vector Map

This post categorized under Vector and posted on September 20th, 2019.

Vector database is a digital collection of vector backbones graphicembled from publications and commercially available sources. This is a get resource for the scientific community that is compiled by Addgene . The pET-28a-c() vectors carry an N-terminal HisTag thrombinT7Tag conguration plus an optional C-terminal HisTag sequence. Unique sites are shown on the circle map. Name pET28a Description Bacterial expression vector with T7lac promoter adds N-terminal His tag thrombin cleavage site internal T7 epitope tag C-terminal His tag kanamycin resistance restriction enzyme cloning.

DNASU Plasmid Repository PSIBiology-Materials Repository The Biodesign InsgraphicuteArizona State University 1001 S. McAllister Ave Tempe AZ 85287-6401 Map The pET-28a-c() vectors carry an N-terminal HisTag thrombinT7Tag configuration plus an optional C-terminal HisTag sequence. Note that the sequence is numbered by the pBR322 convention so the T7 expression region is reversed on the vector map ( TB074 ). circular map. The cloningexpression region of the coding strand transcribed by T7 RNA poly- The cloningexpression region of the coding strand transcribed by T7 RNA poly- merase is shown below.

The translation vector names are distinguished from the transcription vector names by the addition of a letter suffix following the name e.g. pET-21a() which denotes the reading frame relative to the Bam H I cloning site recognition sequence GGATCC. Unique sites are shown on the circle map. Note that the sequence is numbered by the pBR322 con- Note that the sequence is numbered by the pBR322 con- vention so the T7 expression region is reversed on the circle map. The pET-20b() vector (Cat. No. 69739-3) carries an N-terminal pelB signal sequence for potential periplasmic localization plus optional C-terminal HisTag sequence. Unique sites are shown on the circle map. Note that the sequence is numbered by the pBR322 convention so the T7 expression region is reversed on the circular map. The cloningexpression region of the coding strand The pET-22b() vector (Cat. No. 69744-3) carries an N-terminal pelB signal sequence for potential periplasmic localization plus optional C-terminal HisTag sequence. Unique sites are shown on the circle map. Note that the sequence is numbered by the pBR322 convention so the T7 expression region is reversed on the circular map. The cloningexpression region of the coding strand

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PET28 Vector Map: Structure Of Recombinant Pet Si Prokaryotic Expression Plasmid And Restrictionfig

Structure Of Recombinant Pet Si Prokaryotic Expression Plasmid And Restrictionfig

Recombinant protein expression varies widelyfrom investigation of function in vivo to large-scale production for structural studies and biotherapeu [more]

PET28 Vector Map: Maps Of Cloning And Expression Vectors A Pgem Zpelb Cloning Vector B Petigtagfig

Maps Of Cloning And Expression Vectors A Pgem Zpelb Cloning Vector B Petigtagfig

The vector give a basic introduction to plasmid vectors. T-Overhangs for Easy PCR Cloning The pG [more]

PET28 Vector Map: Pet Mhl Vector Structural Genomics Consortium

Pet Mhl Vector Structural Genomics Consortium

The pET15-MHL vector (GenBank ID EF456738.1) is a derivative of pET15b (Novagen) and is for infusion based cloning. It is used for [more]

PET28 Vector Map: A Deep Dive Into Iptg Induction

A Deep Dive Into Iptg Induction

Viele bersetzte Beispielstze mit deep dive into something Deutsch-Englisch Wrterbuch und Suchmaschine fr Millionen von Deutsch-ber [more]

PET28 Vector Map: Cloning And Purification Of Nonamer Binding Domain Of Rag A The Nonamer Bindingfig

Cloning And Purification Of Nonamer Binding Domain Of Rag A The Nonamer Bindingfig

Structure determination and refinement. Based on previous studies that established the approximate boundaries of the RAG1 NBD18 19 [more]

PET28 Vector Map: Vector Nti Exercises September Th And Th

Vector Nti Exercises September Th And Th

Vector NTI is a commercial bioinformatics software package used by many life scientists to work among other things with nucleic acids and proteins [more]

PET28 Vector Map: Figure S Schematic Map Of The Expression Vector Pet Cpoi Used For The Cloning Of Thefig

Figure S Schematic Map Of The Expression Vector Pet Cpoi Used For The Cloning Of Thefig

An expression vector otherwise known as an expression construct is usually a plasmid or virus designed for gene expression in cells [more]

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